Genomics Platform

Illumina NextSeq 500

Here are the different options available:

Specifications for 1 slide

Illumina Kit Name	Nb of bp sequenced	Maximum nb of reads (fragments)
NextSeq 75 cycles High Output v2	75	400 M
NextSeq 150 cycles High Output v2	150	400 M
NextSeq 150 cycles Mid Output v2	150	130 M
NextSeq 300 cycles Mid Output v2	300	130 M

All NextSeq kits can be used for single read or paired-end sequencing.

Example:

If you use the NextSeq 150 cycles High Output v2 for paired-end sequencing, you will get 400M reads of 75bp from one end (Read1), and 400M reads of 75bp from the opposite end (Read2) of each fragment.

Applications :

• RNASeq
• Transcriptome
• Amplicons
• Small RNAs (miRNAs)
• Exome
• Targeted genomic regions
• ChIP (chromatin immunoprecipitation)
• Genome re-sequencing

Illumina MiSeq

Here are the different options on the MiSeq:

Illumina Kit Name	Nb of bp sequenced	Maximum nb of reads
MiSeq 300 cycles Nano v2	300	1 M
MiSeq 500 cycles Nano v2	500	1 M reads
MiSeq 300 cycles Micro v2	300	4 M reads
MiSeq Reagent Kit v2 (50-cycle)	50	15 M reads
MiSeq Reagent Kit v3 (150-cycle)	150	25 M reads
MiSeq Reagent Kit v2 (300-cycle)	300	15 M reads
MiSeq Reagent Kit v2 (500-cycle)	500	15 M reads
MiSeq Reagent Kit v3 (600-cycle)	600	25 M reads

All MiSeq kits can be used in paired end.
Number of reads is the number of single-end reads or once the paired-ends are assembled.

Amplicon sequencing

If sequencing a library of amplicons, we do not guarantee the full potential of the MiSeq slide the first time you sequence with us. In addition, we might have to add some PhiX is the diversity of the library is too low.

For amplicon sequencing, we work with Nextera indexing, please contact us prior to your amplification.

Applications :

• RNASeq
• Transcriptome
• Amplicons
• Small RNAs (miRNAs)
• Exome
• Targeted genomic regions
• ChIP (chromatin immunoprecipitation)
• Genome re-sequencing