Next-Generation Sequencing Service
The Genomics Core Facility of IRIC offers Next-Generation Sequencing Services using
Illumina technology featuring unparalleled throughput (hundreds of gigabases) and
accuracy. Select the instruments to view the various applications available.
Illumina NextSeq 2000
Many sequencing options are available, please contact us for all details!
Applications :
- RNASeq
- Amplicons
- Genomes
- Small RNAs
- ChIPseq
- Cut&Tag
- Cut&Run
- Exomes
Illumina NextSeq 500
Here are the different options available:
Specifications for 1 slide
| Illumina Kit Name | Nb of bp sequenced | Maximum nb of reads (fragments) |
|---|
| NextSeq 75 cycles High Output v2 | 75 | 400 M |
| NextSeq 150 cycles High Output v2 | 150 | 400 M |
| NextSeq 150 cycles Mid Output v2 | 150 | 130 M |
| NextSeq 300 cycles Mid Output v2 | 300 | 130 M |
All NextSeq kits can be used for single read or paired-end sequencing.
Example:
If you use the NextSeq 150 cycles High Output v2
for paired-end sequencing, you will get 400M reads of
75bp from one end (Read1), and 400M reads of 75bp
from the opposite end (Read2) of each fragment.
Applications :
- RNASeq
- Transcriptome
- Amplicons
- Small RNAs (miRNAs)
- Exome
- Targeted genomic regions
- ChIP (chromatin immunoprecipitation)
- Genome re-sequencing
Illumina MiSeq
Here are the different options on the MiSeq:
| Illumina Kit Name | Nb of bp sequenced | Maximum nb of reads |
|---|
| MiSeq 300 cycles Nano v2 | 300 | 1 M |
| MiSeq 500 cycles Nano v2 | 500 | 1 M reads |
| MiSeq 300 cycles Micro v2 | 300 | 4 M reads |
| MiSeq Reagent Kit v2 (50-cycle) | 50 | 15 M reads |
| MiSeq Reagent Kit v3 (150-cycle) | 150 | 25 M reads |
| MiSeq Reagent Kit v2 (300-cycle) | 300 | 15 M reads |
| MiSeq Reagent Kit v2 (500-cycle) | 500 | 15 M reads |
| MiSeq Reagent Kit v3 (600-cycle) | 600 | 25 M reads |
All MiSeq kits can be used in paired end.
Number of reads is the number of single-end reads or once the paired-ends are assembled.
Amplicon sequencing
If sequencing a library of amplicons, we do not guarantee the full potential of the MiSeq slide the first time you sequence with us. In addition, we might have to add some PhiX is the diversity of the library is too low.
For amplicon sequencing, we work with Nextera indexing, please contact us prior to your amplification.
Applications :
- RNASeq
- Transcriptome
- Amplicons
- Small RNAs (miRNAs)
- Exome
- Targeted genomic regions
- ChIP (chromatin immunoprecipitation)
- Genome re-sequencing
Documentation :
For a consultation about your Next-Generation Sequencing project, please contact:
nextgen@iric.ca
For inquiries about bioinformatics analysis, contact:
bioinformatique@iric.ca
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